Summary of Study ST002096
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001328. The data can be accessed directly via it's Project DOI: 10.21228/M82978 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002096 |
Study Title | Addressing batch effects in large-scale metabolomics with augmented experimental design and meta-analysis (Part 2) |
Study Summary | Non-polar and polar sequential extracts were collected on three groups of C. elegans: natural isolates, central metabolism mutants, and UDP-glucuronosyltrasnferase mutants. Untargeted LC-MS (HILIC/RP - positive and negative mode) and NMR (CDCl3/H2O) studies were conducted using a Vanquish UHPLC coupled to an Orbitrap Elite MS and NEO 800 MHz Bruker NMR spectrometer, respectively. An augmented design, rank transformation of the raw data, strict feature filtering and QA/QC followed by a meta-analysis was performed on all datasets to demonstrate the benefits of this analysis approach and identify stable, significant features to prioritize for downstream compound identification efforts. |
Institute | University of Georgia - Complex Carbohydrate Research Center |
Laboratory | Edison Lab |
Last Name | Garcia |
First Name | Brianna |
Address | 315 Riverbend Road, Athens, GA, 30602, USA |
brianna.garcia@uga.edu | |
Phone | 7065424401 |
Submit Date | 2022-03-04 |
Num Groups | 3 |
Total Subjects | 116 |
Study Comments | Three study groups of C. elegans strains were used: central metabolism mutants (CMM), UDP-glucuronosyltransferase (UGT) mutants, and natural isolates. |
Raw Data Available | Yes |
Raw Data File Type(s) | fid |
Analysis Type Detail | NMR |
Release Date | 2022-04-04 |
Release Version | 1 |
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Project:
Project ID: | PR001328 |
Project DOI: | doi: 10.21228/M82978 |
Project Title: | Addressing batch effects in large-scale metabolomics with augmented experimental design and meta-analysis |
Project Summary: | Untargeted LC-MS study conducted using RP and HILIC chromatography on three groups of C. elegans: natural isolates, central metabolism mutants, and UDP-glucuronosyltransferase mutants. An augmented study design, rank transformation of the raw data, strict QA/QC followed by a meta-analysis was used for data analysis. |
Institute: | University of Georgia - Complex Carbohydrate Research Center |
Laboratory: | Edison Lab |
Last Name: | Garcia |
First Name: | Brianna |
Address: | 315 Riverbend Road, Athens, GA 30602 |
Email: | brianna.garcia@uga.edu |
Phone: | 7065424401 |
Funding Source: | U2CES030167 |
Contributors: | Amanda O. Shaver, Goncalo J. Gouveia, Alison M. Morse, Zihao Liu, Carter K. Asef, Ricardo M. Borges, Franklin E. Leach III, Erik C. Andersen, I. Jonathan Amster, Facundo M. Fernández, Arthur S. Edison, and Lauren M. McIntyre |