Summary of Study ST000242
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000195. The data can be accessed directly via it's Project DOI: 10.21228/M8630S This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000242 |
Study Title | Whole unconditioned medium (Defined culture media, M199),Whole M1 medium,Whole M2 medium |
Study Type | differential metabolomics |
Study Summary | Media was flash frozen with N2 and stored at -80 C. Samples can be stored at -80 C until use. ~1 ml aliquots. The following media has been provided to the metabolomics core in biological triplicates. Complete (Whole) Media: 1) Whole unconditioned medium (Defined culture media, M199) 2) Whole M1 medium 3) Whole M2 medium |
Institute | Mayo Clinic |
Department | Physiology and Biomedical Engineering |
Last Name | Farrugia |
First Name | Gianrico |
Dasari.Surendra@mayo.edu | |
Submit Date | 2015-06-26 |
Num Groups | 1 |
Raw Data Available | Yes |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2016-07-08 |
Release Version | 2 |
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Project:
Project ID: | PR000195 |
Project DOI: | doi: 10.21228/M8630S |
Project Title: | Metabolimic Analysis of Conditioned Macrophage Media |
Project Type: | Untargeted LC-MS Metabolomics |
Project Summary: | We are studying the secreted components from pro-inflammatory macrophages, coined M1. We use a macrophage at the opposite end of the macrophage phenotype spectrum as a control, the anti-inflammatory macrophage, coined M2. M1 macrophage conditioned media has a <3 kD factor that reduces survival of a cell of interest in primary culture. This factor is not present in M2 macrophage conditioned media <3 kD, as there is no effect on primary cell numbers. The goal of this project is to analyze the components present in the M1 conditioned <3 kD media and compare to the M2 conditioned < 3kD media. |
Institute: | Mayo Clinic |
Department: | Physiology and Biomedical Engineering |
Last Name: | Farrugia |
First Name: | Gianrico |
Address: | 200 First Street SW, Rochester, MN 55905 |
Email: | Dasari.Surendra@mayo.edu |
Phone: | 507-284-0513 |