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MB Sample ID: SA005727
Local Sample ID: | PLO_Met38 |
Subject ID: | SU000123 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 40-76 |
Weight Or Weight Range: | Normal, Overweight, Obese |
Gender: | male , female |
Human Smoking Status: | former |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000120 |
Sampleprep Summary: | Aliquots of plasma samples (630 µL) were transferred into labeled tubes for experimental samples. Pooled high methylatiion, low methylation and combined plasma samples were prepared to serve as external QC samples. For all samples 70 µL of 10 mM formate solution (chemical shift indicator) in 0.9% saline in D2O with 0.02% NaN3 was added to each tube and were vortexed for 30 sec then centrifuged at 12000 rcf for 5 min. A 600 µL aliquot of the supernatant was transferred into 5 mm NMR tubes, which were kept on ice until data acquisition. |
Processing Storage Conditions: | 4°C |
Extract Storage: | -80C |
Sample Resuspension: | D2O Saline |
Sample Spiking: | formate |