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MB Sample ID: SA006157
Local Sample ID: | Stationary phase-P3 |
Subject ID: | SU000135 |
Subject Type: | Bacterial cells |
Subject Species: | Acidipropionibacterium acidipropionici |
Taxonomy ID: | 1748 |
Genotype Strain: | GCMCC1.2230/WSH1105 |
Species Group: | Microorganism |
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Sample Preparation:
Sampleprep ID: | SP000132 |
Sampleprep Summary: | After quenching at 40°C, the cells were pelleted in a centrifuge (4,000 × g, ?4°C, 10 min). The pellets were washed with methanol solution and resuspended in 1 mL 35% perchloric acid to extract the metabolites from the cells. The mixture was frozen in liquid nitrogen and thawed three times. The supernatant was neutralized by adding K2CO3 solution with an initial concentration of 5 M, and an extract containing all metabolites was collected via centrifugation at 10,000 × g for 5 min at 4°C |
Processing Method: | Homogenization and solvent removal |
Processing Storage Conditions: | on ice |
Extraction Method: | 35% perchloric acid and 5 M K2CO3 |
Extract Storage: | -80°C |
Cell Type: | wildtype cells, genome shffled cells |