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MB Sample ID: SA008730
| Local Sample ID: | VT_130304_048 |
| Subject ID: | SU000182 |
| Subject Type: | Human/animal |
| Subject Species: | Homo sapiens;Callithrix jacchus |
| Taxonomy ID: | 9606;9483 |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000182 |
| Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Vortex for 1 minute on high and leave on ice for 30 minutes. Centrifuge for 10 minutes at 13.2rpm. 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Processing Method: | precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | supernatant pipette into autosampler vials for mass spectrometer |
| Sample Spiking: | internal standards: [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, 2'-deoxyguanosine-13C10,15N5-5'-monophosphate |
