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MB Sample ID: SA010722
Local Sample ID: | SF-100109-259700 |
Subject ID: | SU000239 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Cell Biosource Or Supplier: | Moffitt Cancer Center Tissue Core |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000241 |
Sampleprep Summary: | Pulverized tissue samples were incubated in 50:50 Acetonitrile:Water for 1 hr, vortexed and centrifuged. Supernatants were transferred to new tubes along with isotopocially labled internal standard and samples were lyophilized to dryness. Dried samples were resuspended in 95:5 Water:Methanol and an aliquot was transferred to autosampler vials forbroad spectrum metabolomics data analysis acquisition. |
Processing Method: | Pulverization of frozen tissue (performed at Moffitt and supplied to RTI) |
Extraction Method: | 50:50 Acetonitrile:Water |
Extract Storage: | -80 C |
Sample Resuspension: | 95:5 Water:Methanol |
Sample Spiking: | L-Tryptophan-d5 |
Organ: | Lung |