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MB Sample ID: SA010758
| Local Sample ID: | SVM0420 |
| Subject ID: | SU000242 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000244 |
| Sampleprep Summary: | Aliquots of plasma samples (125 µL) were transferred into pre-labeled 2.0 mL low-bind Eppendorf tubes for experimental samples. For all samples 125 µL of 0.9% Saline in D2O (containing 2 mM Formate (chemical shift indicator) and 0.2% NaN3 (to prevent bacterial growth)) was added to each tube and were vortexed for 2 min at 5000 rpm. The samples were then centrifuged at 16,000 rcf for 2 min. Total pools of the samples were prepared (by using 12 µL of each study sample) to serve as QC samples, and 125 µL aliquots of QC samples were processed in the same way as described above for study samples. A 200 µL aliquot of the samples were then transferred into 3 mm NMR tubes (Bruker-Biospin, Switzerland), which were kept in a cooler with freeze packs until data acquisition. |
| Processing Storage Conditions: | 4°C |
| Sample Resuspension: | 0.9% Saline in D2O |
| Sample Spiking: | Formate |
