Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA010879

Local Sample ID:	CON4
Subject ID:	SU000251
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57BL/6
Age Or Age Range:	6 weeks at collection
Gender:	Female
Animal Animal Supplier:	Jackson Labs
Animal Housing:	Conventional
Animal Feed:	Low Protein, Low Fat Malnourished Diet\|Isocaloric Control Diet
Subject Comments:	2 subjects
Cell Primary Immortalized:	Malnourished\|Control
Species Group:	Mammal

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Sample Preparation:

Sampleprep ID:	SP000255
Sampleprep Summary:	Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS).

Sampleprep ID:

SP000255

Sampleprep Summary:

Metabolite Extraction. Mouse small intestine digesta samples (luminal contents) were collected, weighed and homogenized in water followed by addition of acetonitrile for metabolite extraction. After vortex mixing, 30 second sonication, and centrifugation, the clear supernatants were collected and dried under a gentle nitrogen gas flow. The dried metabolite residues were dissolved in 40% acetonitrile containing 0.01% formic acid, 4 ?L per mg of the raw material. After 15 sec vortex and 30 sec sonication, each sample was further diluted 1:2 with water. Twelve-?L aliquots were injected for untargeted metabolic fingerprinting by ultrahigh-performance liquid chromatography ? Fourier transform mass spectrometry (UPLC-FTMS).