Return to study ST000254 main page
MB Sample ID: SA011574
| Local Sample ID: | P7 |
| Subject ID: | SU000274 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000280 |
| Sampleprep Summary: | Aliquots of plasma samples (20 µL) were transferred into pre-labeled 2.0 mL low-bind Eppendorf tubes for experimental samples. For all samples 100 µL Protein Precipitation Buffer with internal standard [2.5 ng/ml of 2-methyl-4-chlorophenyl-acetic acid (MCPA) in methanol] was added to each tube and were vortexed for 2 min at 5000 rpm. The samples were centrifuged at 16,000 rcf for 4 min. A 90 µL of the supernatant wastransferred into pre-labeled auto-sampler vials and diluted by adding 90 µL of water with 10% Formic Acid. Samples were then mixed on a multiple tube vortexer for 5 min at 5000 rpm. 10 µL of samples were injected onto API-4000 Q-trap. |
| Processing Storage Conditions: | 4°C |
| Extract Storage: | -80C |
| Sample Resuspension: | Methanol, H2O, formic acid |
| Sample Spiking: | 2-methyl-4-chlorophenyl-acetic acid (MCPA) |
