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MB Sample ID: SA012087
Local Sample ID: | QE1_REP_18_11 |
Subject ID: | SU000289 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57BL/6 |
Age Or Age Range: | 8 or 24 week |
Gender: | male |
Animal Animal Supplier: | Jackson Laboratories |
Animal Feed: | Control (Research Diets, Inc. # D09100304) and trans-fat diet (Research Diets, Inc. # D09100301) |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000297 |
Sampleprep Summary: | Tissues were cryogenically ground prior to beign weighed and homogenized in chloroform and methanol for the Folch extraction |
Processing Method: | homogenization (zirconium beads, 1.0 mm diameter), lipid extraction |
Processing Storage Conditions: | Liquid nitrogen and on ice |
Extraction Method: | Folch |
Extract Storage: | -80C |
Sample Resuspension: | isopropanol |
Sample Spiking: | Internal Standards: Cer(d18:1/17:0) and d5-DAG (20:2/20:2) and d5-DG (20:5/20:5) |
Organ: | liver |