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MB Sample ID: SA013847
Local Sample ID: | DP2S138 |
Subject ID: | SU000326 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Gender: | male/female |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000334 |
Sampleprep Summary: | Plasma samples were transferred to labeled tubes. A total of 219 study samples were thawed on ice for sample preparation200 uL of plasma sample were thawed and transferred to labeled tubes on ice where they were mixed with 50 uL Saline master mix (5mM Formate). Analytical quality control (QC) phenotypic pooled samples were generated by transferring a 25 µL of each sample of each respective phenotypical experimental sample into different 1.5 mL tubes. Whole study (total) pools were generated by transferring 200 uL of plasma from each Pool sample into a 2.0 mL tube. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 200 µl aliquot of the supernatant was transferred into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer. |
Sampleprep Protocol Filename: | CAD_Metabolomics_Procedures.docx |