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MB Sample ID: SA014448
Local Sample ID: | HFCLM1 |
Subject ID: | SU000336 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000344 |
Sampleprep Summary: | For blood collection, the mice were sacrificed by cardiac puncture. Plasma (100 µL) was subjected to extraction with cold methanol (500 µL) on a MM400 mixer mill (Retsch®, Haan, Germany) with the frequency 30 s?1 for 5 min. The suspension was centrifuged at 12,578 g for 10 min at 4°C. The supernatant was filtered through a 0.2-µm PTEE filter and evaporated in a speed vacuum concentrator. The final concentration of the plasma was 5 mg/mL in methanol for the UPLC-Q-TOF-MS analysis. |