Return to study ST000359 main page
MB Sample ID: SA015981
Local Sample ID: | Micro438 |
Subject ID: | SU000380 |
Subject Type: | mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57Bl/6 |
Age Or Age Range: | 5.5 mo |
Weight Or Weight Range: | 29.1 g-49.2 g |
Gender: | male |
Animal Animal Supplier: | JAX |
Animal Housing: | 4/cage |
Animal Feed: | Purina chow |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP000387 |
Sampleprep Summary: | For extraction, 500 µL of an ice-cold solution of 90:10 Methanol:Chloroform (v/v) ¬was added to the tubes containing the thawed cell pellets. MagNA lyser ceramic beads were added to the tubes, and a MagNA lyser was used to beat the samples for two 30 seconds pulses at 2,000 rpm placing samples on cold block for 5 min in between pulses. Sonication for 5 min was performed to the beaten cell samples, followed by centrifugation at room temperature and at 16,000rcf for 4 min. A 350 µL aliquot of each experimental cell sample homogenized supernatant was transferred to a labeled 2.0 mL Lo-Bind Eppendorf tube Analytical pooled samples were created by combining a 125 µL aliquot from each experimental sample in a labeled 20 mL glass vial. The QC pooled sample was vortexed for 30 sec and 350 µL aliquots were transferred to 5 labeled 2.0 mL Lo-Bind eppendorf tubes. Next, the supernatants of the study and QC pooled samples were dried overnight using a lyophilizer. The residue was reconstituted in 50 µL of reconstitution buffer (95:5 Water:Methanol, v/v) and mixed on an analog vortex mixer for 1 min with speed set at 10. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and the supernatants were transferred to autosampler vials for data acquisition. |