Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA016505

Local Sample ID:	CC0908
Subject ID:	SU000387
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Gender:	Male and Female
Human Race:	African American
Species Group:	Human

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Sample Preparation
Analysis
NMR

Sample Preparation:

Sampleprep ID:	SP000394
Sampleprep Summary:	Fecal samples (20 mg) were mixed with 1000 μL of D2O, mixed thoroughly by vortexing, and then centrifuged at 16000 x g for 20 min. The supernatants were removed and filtered through 0.22μ centrifuge filters at 16000 x g for 20 minutes. 540 μl of the filtrate was mixed with 60 μl of Chenomix Internal Standard mixture (containing DSS, Imidazole, and NaN3 in D2O). Aliquots of 550 μl were then transferred into 5mm NMR tubes.

Sampleprep ID:

SP000394

Sampleprep Summary:

Fecal samples (20 mg) were mixed with 1000 μL of D2O, mixed thoroughly by vortexing, and then centrifuged at 16000 x g for 20 min. The supernatants were removed and filtered through 0.22μ centrifuge filters at 16000 x g for 20 minutes. 540 μl of the filtrate was mixed with 60 μl of Chenomix Internal Standard mixture (containing DSS, Imidazole, and NaN3 in D2O). Aliquots of 550 μl were then transferred into 5mm NMR tubes.