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MB Sample ID: SA018020
| Local Sample ID: | Sarz195_51245pre_085_CSHMS.d |
| Subject ID: | SU000408 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000415 |
| Sampleprep Summary: | 1) Degas both “MeOH with QCmix” and MTBE with Cholesterol Ester 22:1 bysonication 2) Store solvents in the –20°C freezer to pre-chill 3) Thaw each 20 μL plasma aliquot at room temperature. Once thawed (~10min) place liquid plasma samples on ice. 4) Add 225 μL cold “MeOH with QC mix” (see SOP “QC mix for LC-MS lipid analysis”). Keep MeOH on ice during extraction 5) Vortex each sample for 10s, keeping the rest on ice during all the extraction. 6) Add 750 μL of cold MTBE with 22:1 CE, keep MTBE on ice during extraction Vortex for 10s 7) Shake for 6min at 4°C in the orbital mixer. Add 188 μL room temperature LC/MS grade water. 8)Vortex for 20 s 9) Centrifuge for 2 min @ 14,000 rcf (12300 rpm) 10) Remove supernatant, splitting into two aliquots of 350 μL, keeping one at –20°C for backup 11) Dry samples to complete dryness in the speed vacuum concentration system |
| Sampleprep Protocol Filename: | SOP_Lipid_Extraction_Plasma_06242013 (1).pdf |
| Processing Method: | Homogenization |
