Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA019987

Local Sample ID:	F_15
Subject ID:	SU000430
Subject Type:	Mice
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Sample Preparation:

Sampleprep ID:	SP000437
Sampleprep Summary:	Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000437

Sampleprep Summary:

Fecal pellets were transferred into a pre-labeled MagnaLyser tube and massed. Phosphate buffer was added depending on the mass of each sample (500 uL for up to 100 mg and 1000 uL for more than 200 mg). Samples were homogenized using MagnaLyser for two 30 sec pulses and then centrifuged. The supernatant of each sample was transferred into pre-labeled 0.2 um nylon filter tube and centrifuged. A volume of each filtrate required to analyze 25 mg of sample was aliquoted, mixed with a volume of phosphate buffer to bring the total volume to 630 uL, and 70 uL of Chenomx ISTD. For a subset of samples with excess material, a total study pool was created by mixing the remaining volume. Five replicates were created, and these prepared identically to the study samples. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 4 min. A 600 µl aliquot of the supernatant was transferred into pre-labeled 5 mm (4”) NMR tubes for data acquisition on a 700 MHz spectrometer.