Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA022939

Local Sample ID:	CE_AA_NO_1_20_C
Subject ID:	SU000474
Subject Type:	Bacterial Cells
Subject Species:	Staphylococcus aureus
Taxonomy ID:	1280
Species Group:	Microorganism

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Sample Preparation:

Sampleprep ID:	SP000481
Sampleprep Summary:	Methylillin-resistant S. aureus (MRSA) bacterial culture extracts re-suspended in water were provided to RTI RCMRC for analysis. These extracts were stored at -80 °C upon arrival. For processing, samples were dried overnight on a lyophilizer. The residue was then reconstituted with 150 µL of an ice-cold solution of reconstitution buffer (0.005 mg/mL Tryptophan-d5 in 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 2 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and 100 µL of the supernatants were transferred to autosampler vials for data acquisition. Whole study pooled QC samples were created by combining 40 µL aliquot from each of the reconstituted samples into a 2 mL LoBind eppendorf tube. The QC pooled sample was then vortexed for 15 sec and 100 µL of the total study pooled QC samples were transferred to five pre-labeled autosampler vials.

Sampleprep ID:

SP000481

Sampleprep Summary:

Methylillin-resistant S. aureus (MRSA) bacterial culture extracts re-suspended in water were provided to RTI RCMRC for analysis. These extracts were stored at -80 °C upon arrival. For processing, samples were dried overnight on a lyophilizer. The residue was then reconstituted with 150 µL of an ice-cold solution of reconstitution buffer (0.005 mg/mL Tryptophan-d5 in 95:5 Water:Methanol, v/v) and mixed on a multiple tube vortexer for 2 min at 5,000 rpm. Then, the samples were centrifuged at room temperature for 4 min at 16,000 rcf, and 100 µL of the supernatants were transferred to autosampler vials for data acquisition. Whole study pooled QC samples were created by combining 40 µL aliquot from each of the reconstituted samples into a 2 mL LoBind eppendorf tube. The QC pooled sample was then vortexed for 15 sec and 100 µL of the total study pooled QC samples were transferred to five pre-labeled autosampler vials.