Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA023067

Local Sample ID:	M_3
Subject ID:	SU000476
Subject Type:	Human Cell Media
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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NMR

Sample Preparation:

Sampleprep ID:	SP000483
Sampleprep Summary:	Cell media was extracted with 50% acetonitrile:water, vortexed, and centrifuged. An analytical quality control (QC) total pool was generated from equal aliquots from all of the media samples, divided into three total pool aliquots, and processed identically to the media study samples. All study and pool samples were lyophilized to dryness and reconstituted in a 0.2M phosphate buffer, pH 7.4, in D2O with 10% Chenomx ISTD and 6mM Imidazole.

Sampleprep ID:

SP000483

Sampleprep Summary:

Cell media was extracted with 50% acetonitrile:water, vortexed, and centrifuged. An analytical quality control (QC) total pool was generated from equal aliquots from all of the media samples, divided into three total pool aliquots, and processed identically to the media study samples. All study and pool samples were lyophilized to dryness and reconstituted in a 0.2M phosphate buffer, pH 7.4, in D2O with 10% Chenomx ISTD and 6mM Imidazole.