Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA023291

Local Sample ID:	B_36
Subject ID:	SU000483
Subject Type:	Murine
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000490
Sampleprep Summary:	Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000490

Sampleprep Summary:

Brain tissue samples were homogenized in 50:50 Acetonitrile:Water , and the final concentration of all of the extracts was 1 mL/mg. Of the 71 samples, 48 had sufficient volume for study samples and for an analytical quality control (QC) total pool sample. This total pool was generated by transferring a 50 µL of each sample and aliquoting into six replicates, which were processed identically to the study samples. A 250 µL aliquot of each sample homogenate was lyophilized and then reconstituted with 250 µL of Phosphate buffer with Chenomx ISTD and 6mM Imidazole. Samples were vortexed and centrifuged before transferring 225 µL of each sample into pre-labeled 3mm NMR tubes for data acquisition on a 700 MHz spectrometer.