Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA023433

Local Sample ID:	P_14
Subject ID:	SU000485
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Sample Preparation:

Sampleprep ID:	SP000492
Sampleprep Summary:	De-identified samples were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. Study samples were thawed on ice for sample preparation. A 150 µL aliquot of plasma was transferred to new labeled tubes for each study sample. A total study pool was generated by transferring 15 µL of plasma from each sample into a new 10 mL Falcon tube. The total pool sample was vortexed and 150 µL aliquots were transferred into 10 total pool-labeled tubes. Plasma and pooled samples were extracted with 450 µL methanol, vortexed for 2 min on a multi-tube vortexer, and centrifuged at 16,000 rcf for 5 min. A 400 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and the supernatant was lyophilized to complete dryness overnight. Samples were reconstituted with 250 µL of NMR master mix solution containing Chenomx ISTD: DSS-d6 and 0.20 M phosphate buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 200uL aliquot of supernatants were transferred into a pre-labeled 3mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000492

Sampleprep Summary:

De-identified samples were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. Study samples were thawed on ice for sample preparation. A 150 µL aliquot of plasma was transferred to new labeled tubes for each study sample. A total study pool was generated by transferring 15 µL of plasma from each sample into a new 10 mL Falcon tube. The total pool sample was vortexed and 150 µL aliquots were transferred into 10 total pool-labeled tubes. Plasma and pooled samples were extracted with 450 µL methanol, vortexed for 2 min on a multi-tube vortexer, and centrifuged at 16,000 rcf for 5 min. A 400 µl aliquot of the supernatant was transferred into pre-labeled 2.0mL LoBind Eppendorf tubes, and the supernatant was lyophilized to complete dryness overnight. Samples were reconstituted with 250 µL of NMR master mix solution containing Chenomx ISTD: DSS-d6 and 0.20 M phosphate buffer at 7.4 pH. The tubes were vortexed for 2 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 200uL aliquot of supernatants were transferred into a pre-labeled 3mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.