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MB Sample ID: SA027879

Local Sample ID:S_60
Subject ID:SU000552
Subject Type:Serum
Subject Species:Mus musculus
Taxonomy ID:10090
Genotype Strain:C57BLKsJ-db/db (disease model) and C57BLKsJ wild type (wild type)
Age Or Age Range:14 weeks; drug administration since the 5th week
Weight Or Weight Range:37-58 g for db/db mice; 18-25 g for wild type mice
Gender:Male
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000559
Sampleprep Summary:In lipidomics analysis, samples were first thawed on ice, and then mixed by vortex for 4 min at 4,000 rpm, followed by centrifugation for 4 min at 4, 000 rcf to spin down particulate. Pooled mouse serum from Sigma Aldrich (S7273) were used as QC samples. A 30 µL aliquot of each serum sample (including QC sample and real sample) was transferred to a 2.0 mL Eppendorf Lo-Bind tube and extracted by 600 µL of 2:1 dichloromethane: methanol (DCM) via vortex-mixing for 2 min at 4,000 rpm. Then, 120 µL of H2O was added and repeated the vortex-mixing for 1 min at 4,000 rpm. After placing the sample at room temperature for 10 min, the tube was centrifuged at 16,000 rcf for 10 minutes at 10 °C. A 370 µL aliquot of the lower lipid-rich DCM layer was transferred to a new labeled 2.0 mL Eppendorf Lo-Bind tubes and then lyophilized to complete dryness overnight. For immediate analysis, 300 µL of acetonitrile:isopropanol:H2O (65:30:5, v/v/v) was added to reconstitute the dried tissue extract, and the samples were thoroughly mixed on a multi-tube vortexer for 10 min at 5000 rpm and centrifuged at 16,000 rcf for 4 min. The supernatants were transferred to labeled autosampler vials for data acquisition by LC-MS.
Extraction Method:cold 2:1 dichloromethane: methanol vortex extraction
Extract Storage:-80 oC
Sample Resuspension:acetonitrile:isopropanol:H2O (65:30:5, v/v/v)
Sample Spiking:1,2-diheptadecanoyl-sn-glycero-3-phosphocholine
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