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MB Sample ID: SA029135
| Local Sample ID: | M79 |
| Subject ID: | SU000587 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Gender: | Both |
| Human Trial Type: | validation/discovery trial |
| Human Inclusion Criteria: | All subjects will receive hemodialysis in-center on a 3 times per week dialysis schedule. Subjects are eligible to participate if 1) subjects that have received EPO will have received it for 6 or more months, 2) between the ages of 18 and 80 years, 3) have an adequacy of dialysis (Kt/V) that is ≥ 1.2, 4) have adequate iron stores as measured by ferritin and transferrin saturation. |
| Human Exclusion Criteria: | None |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000594 |
| Sampleprep Summary: | A volume of 150 µL of each EDTA-plasma sample was dispensed into an acid-washed plastic digestion tube. To each digestion vessel, 300 µL of concentrated HNO3, 150 µL of concentrated HCl, and 100 µL of H2O2 solution was added. Samples were loosely capped and placed in the graphite digestion block and heated at a temperature of 90 °C for 2 hours. At the end of digestion, all samples were removed from the heating block and allowed to cool to room temperature. In the clean hood, samples were spiked with a multielement internal standard to provide a final concentration of 1.0 ng/mL Sc, In, and Pr, and diluted to the final volume with deionized (DI) H2O. |
| Processing Method: | Acid Digestion |
| Processing Storage Conditions: | 90°C |
| Extract Storage: | 8°C |
| Sample Spiking: | Sc, In, Pr |
