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MB Sample ID: SA029905
Local Sample ID: | 140505aaasa11_1 |
Subject ID: | SU000591 |
Subject Type: | Microorganisms |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
Species Group: | Microorganism |
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Sample Preparation:
Sampleprep ID: | SP000598 |
Sampleprep Summary: | metabolite samples were derivatized in two steps. First, the samples were incubated with 5 µl of 40 mg/ml methoxyamine hydrochloride in pyridine (Sigma-Aldrich, St. Louis, MO) at 30 °C for 90 min, and then, with 45 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) at 37 °C for 30 min. A mixture of fatty acid methyl esters including methyl forms of C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, and C30 was added to the derivatized sample as the retention index markers. |