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MB Sample ID: SA030777
Local Sample ID: | DBA2J 8-8.5M FAH4L3 |
Subject ID: | SU000605 |
Subject Type: | Animal |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | DBA/2J |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000612 |
Sampleprep Summary: | A total of ~3 μl of AH and ∼0.5-1 mm3 of TM per mice was used for phospholipid extraction using modified Bligh and Dyer extraction method[13,14]. Phospholipids extracted in the lower organic phase were dried using a Speed-Vac (Model 7810014; Labconco, Kansas City, MO), and then flushed with argon gas for oxidation prevention. Proteins procured from the upper aqueous phase were quantified using a suitable modification of Bradford’s method. |