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MB Sample ID: SA031330
| Local Sample ID: | nhilic-25nov15-004-r001 |
| Subject ID: | SU000607 |
| Subject Type: | Zebrafish |
| Subject Species: | Danio rerio |
| Taxonomy ID: | 7955 |
| Species Group: | Fish |
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Sample Preparation:
| Sampleprep ID: | SP000614 |
| Sampleprep Summary: | Handling of tissue 1) Keep tubes on ice for 10 min. Spin down @ 1000 RCF for 30 sec 2) Add 20 ul PBS per sample and 3 ul 13C6-Phe 3) Sonicate on ice bath for 30 sec x 4 times 4) Add 250 ul ACN/MeOH mixture and incubate on ice for 30 min 5) Pool 20 ul sup from each sample and split 100 ul pool in 5 tubes 6) Split remaining sup in two 1 dram vials x 2 (100 ul per vial) 7) Dry under N2 stream |
