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MB Sample ID: SA032618
Local Sample ID: | 1932203 |
Subject ID: | SU000615 |
Subject Type: | Animal |
Subject Species: | Macaca mulatta |
Taxonomy ID: | 9544 |
Genotype Strain: | N/A|6.53kg|6.87kg|6.09kg|7.23kg|6.48kg |
Gender: | Male| Male| Male| Male| Male| Male |
Animal Animal Supplier: | none|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center| |Yerkes Primate Research Center |
Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
Animal Light Cycle: | 12/11|12/12|12/12|12/12|12/12|12/12 |
Animal Feed: | Monkey Jumbo Diet 5037 and 5038 |
Animal Water: | tap in bottle |
Animal Inclusion Criteria: | Weight |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP000622 |
Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx |
Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
Processing Storage Conditions: | On ice |
Extraction Method: | 1:2 sample:acetonitrile |
Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Anion Exchange Standards: Cystine, cysteine-glutathione disulfide, carboxymethyl-cysteine, glutathione disulfide, carboxymethyl-glutathione. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |