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MB Sample ID: SA033215
Local Sample ID: | 10182M |
Subject ID: | SU000624 |
Subject Type: | Human Plasma |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 57-70 |
Gender: | Males and Females |
Human Race: | Non-Hispanic White |
Human Smoking Status: | Current and Former Smokers |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000631 |
Sampleprep Summary: | 100µL of plasma was prepared using methanol to extract precipitate the proteins into a pellet. A liquid-liquid extraction with water and methyl-tert butyl ether (MTBE) was used to partition the supernatant into an aqueous layer and an organic layer (lipids). The aqueous layer was dried down in a SpeedVac at 45°C, then reconstituted in 100µL of 95:5 water:acetonitrile. The lipid fraction was dried down in a SpeedVac at 45°C, then reconstituted in 200µL of 100% methanol. |