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MB Sample ID: SA033432
Local Sample ID: | CCMB04WB |
Subject ID: | SU000628 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | Healthy - Mean (±SD): 54.9±8.2 years | Sepsis - Mean (+SD): 54.7±17.3 years |
Weight Or Weight Range: | Healthy - Mean (+SD): 74.4±1.71 kg; BMI < 30 |
Height Or Height Range: | Healthy - Mean (±SD): 171.8±10.3cm |
Gender: | Healthy - males and females | Sepsis - males and females |
Human Race: | Healthy - Caucasian (C) and African American (AA) | Sepsis - Caucasian (C) and African American (AA) |
Human Medications: | Healthy - None |
Human Prescription Otc: | Healthy - None |
Human Smoking Status: | Healthy - Non smokers |
Human Alcohol Drug Use: | Healthy - None |
Human Inclusion Criteria: | Healthy - non-smoking, non-obese with no known medical conditions that require chronic drug therapy | Sepsis - admitted to emergency department or intensive care unit. Clinical indices for severe sepsis or septic shock were met: confirmed or suspected infection, two or more criteria of systemic inflammatory response criteria, and hypoperfusion evidenced by either a systolic blood pressure of <90mmHg after 20 min of fluid resuscitation or a blood lactate level of at least 36 mg/dL. |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000635 |
Sampleprep Summary: | Methanol:cholorform extraction, filtration |
Sampleprep Protocol Comments: | Lipid fractions saved, stored at -20°C |
Processing Method: | Methanol:Chloroform Extraction, filtration |
Processing Storage Conditions: | 0-4°C |
Extraction Method: | Methanol:chloroform extraction |
Extract Enrichment: | Ultra-filtration to remove residual protein |
Extract Cleanup: | N/A |
Extract Storage: | -80°C |
Sample Resuspension: | 500 uL Deuterium Oxide (D2O) |
Sample Spiking: | DSS-IS2 (Chenomx, Inc) |