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MB Sample ID: SA034994
Local Sample ID: | 12M_MTM |
Subject ID: | SU000643 |
Subject Type: | Mouse |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000650 |
Sampleprep Summary: | AH and TM samples were subjected to lipid extraction using suitable modification of the Bligh and Dryer method. All extractions were made without addition of any extraneous ions such as lithium or acetate ions unless stated otherwise. Some confirmatory additional experiments were performed, where 1mM LiCl or 1mM ammonium acetate (for positive and negative ion mode analyses, respectively) was added to the aliquot being analyzed just before analyses. The samples were dried with a Speed-Vac and flushed with argon gas. Uniformity of extraction was ensured with an added external standard (1, 2-ditridecanoyl-snglycero-3-phosphocholine) during tissue homogenization. All extractions and subsequent handling were done using glass vials to avoid contaminating impurities. Extracted lipids were dried and resuspended in LC-MS grade Acetonitrile:Isopropanol (1:1). |