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MB Sample ID: SA035213
Local Sample ID: | BALF_MET107_1x_lipid_pos_B |
Subject ID: | SU000650 |
Subject Type: | Human | Animal |
Subject Species: | Homo sapiens;Mus musculus |
Taxonomy ID: | 9606;10090 |
Genotype Strain: | Animal: C57BL/6 |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000657 |
Sampleprep Summary: | 100uL of BALF were prepared using methanol assisted protein precipitation followed by methyl-tert butyl ether (MTBE) liquid-liquid extraction. Both liquid and aqueous fractions were preserved for analysis. The lipid fraction was reconstituted in 200µL of methanol. The lipid fraction was dried down in a SpeedVac at 45°C for reconstitution in 50 µL of 95:5 water:acetonitrile. |