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MB Sample ID: SA049103
Local Sample ID: | A10 |
Subject ID: | SU000899 |
Subject Type: | Sheep |
Subject Species: | Ovis aries |
Taxonomy ID: | 9940 |
Gender: | Both |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000906 |
Sampleprep Summary: | After randomization, a 50 μl plasma aliquot in a 1.5 mL polypropylene Eppendorf tube was enriched with deuterated surrogates in 10 µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 200 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol/acetonitrile (v/v) was added, the sample mixed by vortexing and protein precipitate was removed by centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until UPLC-MS/MS analysis. |