Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA049131

Local Sample ID:	G02
Subject ID:	SU000899
Subject Type:	Sheep
Subject Species:	Ovis aries
Taxonomy ID:	9940
Gender:	Both
Species Group:	Mammal

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000906
Sampleprep Summary:	After randomization, a 50 μl plasma aliquot in a 1.5 mL polypropylene Eppendorf tube was enriched with deuterated surrogates in 10 µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 200 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol/acetonitrile (v/v) was added, the sample mixed by vortexing and protein precipitate was removed by centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until UPLC-MS/MS analysis.

Sampleprep ID:

SP000906

Sampleprep Summary:

After randomization, a 50 μl plasma aliquot in a 1.5 mL polypropylene Eppendorf tube was enriched with deuterated surrogates in 10 µL methanol (Tables S1 and S2 from Agrawal, K., L.A. Hassoun, N. Foolad, T.L. Pedersen, R.K. Sivamani, J.W. Newman. 2017. Sweat lipid mediator profiling: a non-invasive approach for cutaneous research. J. Lipid Res. 58:188–195 [EPub: Nov 7, 2016]. doi: 10.1194/jlr.M071738) and 5 μl of BHT/EDTA in 1:1 methanol/water (v/v). A total of 200 μl 1-cyclohexyl uredio, 3-dodecanoic acid / 1-phenyl ureido, 3-hexanoic acid (CUDA / PUHA) in 1:1 methanol/acetonitrile (v/v) was added, the sample mixed by vortexing and protein precipitate was removed by centrifugation (10 min, 10,000 RCF, 6 °C). The supernatant was filtered by centrifugation through 1 µm PVDF membranes (Millipore, Billerica, MA) at 6 °C and 4,500 RCF for 3 min. The filtrate was stored in glass vials at -20 °C until UPLC-MS/MS analysis.