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MB Sample ID: SA051144
| Local Sample ID: | S11 |
| Subject ID: | SU000915 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 13 - 66 |
| Gender: | Female and Male |
| Human Ethnicity: | Brazillian |
| Human Inclusion Criteria: | Retrospective serum samples from leprosy patients diagnosed or not with type 1 reaction that were not receiving multidrug therapy or corticosteroid therapy at the time of blood collection. |
| Human Exclusion Criteria: | Retrospective serum samples from leprosy patients that were under multidrug therapy and/or corticosteroid therapy at the time of blood collection were excluded from the study. |
| Subject Comments: | The patients exhibited borderline tuberculoid, borderline borderline and borderline lepromatous forms of leprosy and was diagnosed or not with type 1 reaction. |
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Sample Preparation:
| Sampleprep ID: | SP000922 |
| Sampleprep Summary: | Serum samples (7 serum samples from leprosy patients with T1R) were pooled prior the extraction. These pooled serum samples were incubated with 3 volumes of cold methanol (100%) for 1 hour at -20C. After this incubation, the samples were centrifuged for 30 minutes at 18,000 xg at 4C to clear the supernatant. The supernatant was then transferred and dried under vacuum. Samples were re-suspended in 80 μl of 50% methanol. Prior to the injections on the LC-MS instrument, the samples were centrifuged for 30 minutes at 18,000 xg at 4oC. |
| Sampleprep Protocol ID: | SP000100 |
| Sampleprep Protocol Filename: | 060613_Methanol_Serum_Extraction |
| Sampleprep Protocol Comments: | Use always nitrile gloves. Do not use gloves with powder. Use pipette tips with aerosol barrier. |
| Processing Method: | Homogenization and solvent removal with Speed Vac |
| Processing Storage Conditions: | on ice |
| Extraction Method: | Cold Methanol extraction (3:1 methanol/water) |
| Extract Enrichment: | no enrichment was performed |
| Extract Cleanup: | Centrifugation |
| Extract Storage: | -80C |
| Sample Resuspension: | 50% methanol |
| Sample Derivatization: | no derivatization process was performed |
| Sample Spiking: | Samples were spiked with the following commercial standards: arachidonic acid, docosahexaenoic acid, eicosapentaenoic acid and leukotriene B4 |
