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MB Sample ID: SA051152
| Local Sample ID: | S15 |
| Subject ID: | SU000916 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 13 - 66 |
| Gender: | Female and Male |
| Human Inclusion Criteria: | Retrospective serum samples from leprosy patients diagnosed or not with type 1 reaction that were not receiving multidrug therapy or corticosteroid therapy at the time of blood collection. |
| Human Exclusion Criteria: | Retrospective serum samples from leprosy patients that were under multidrug therapy and/or corticosteroid therapy at the time of blood collection were excluded from the study. |
| Subject Comments: | The patients exhibited borderline tuberculoid, borderline borderline and borderline lepromatous forms of leprosy and was diagnosed or not with type 1 reaction. |
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Sample Preparation:
| Sampleprep ID: | SP000923 |
| Sampleprep Summary: | The retrospective serum samples (80 microliters[ul]) were incubated with 3 volumes of cold methanol (100%) for 1 hour at -20C. After this incubation, the samples were centrifuged for 30 minutes at 18,000 xg at 4C to clear the supernatant. The supernatant was then transferred and dried under vacuum. Samples were re-suspended in 80 μl of 50% methanol. Prior to the injections on the LC-MS instrument, the samples were centrifuged for 30 minutes at 18,000 xg at 4oC. |
| Sampleprep Protocol ID: | SP000100 |
| Sampleprep Protocol Filename: | 060613_Methanol_Serum_Extraction |
| Sampleprep Protocol Comments: | Use always nitrile gloves. Do not use gloves with powder. Use pipette tips with aerosol barrier. |
| Processing Storage Conditions: | -80C |
| Extraction Method: | Methanol extraction |
| Extract Cleanup: | Centrifugation |
| Extract Storage: | -80C |
| Sample Resuspension: | 50% methanol |
| Sample Derivatization: | no derivatization process was performed |
