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MB Sample ID: SA051271
Local Sample ID: | 150706bctsa22_2 |
Subject ID: | SU000918 |
Subject Type: | Cells |
Subject Species: | Escherichia coli |
Taxonomy ID: | 562 |
Species Group: | Microorganism |
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Sample Preparation:
Sampleprep ID: | SP000925 |
Sampleprep Summary: | B6 species [PLP, PNP, pyridoxamine 5'-phosphate (PMP), PL, PN and PM] were extracted from WT and ΔyggS pellets (1.0 ml culture of OD60051.0) in 0.9 ml 5 % (w/v) metaphosphoric acid and 0.1 ml internal standard 4-deoxypyridoxine (73 nmol/ml in 5 % metaphosphoric acid). The suspension was vortexed and sonicated. After centrifugation at 10 000 g for 15 min, the supernatant was filtered and a 50 ml sample was taken for HPLC analysis using fluorescence detection (excitation 328 nm, emission 393 nm). |