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MB Sample ID: SA052461
Local Sample ID: | 4633 PB1 |
Subject ID: | SU000927 |
Subject Type: | Animals |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
Genotype Strain: | C57/B6 |
Age Or Age Range: | 8-16 weeks |
Species Group: | Mammal |
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Sample Preparation:
Sampleprep ID: | SP000934 |
Sampleprep Summary: | For free fatty acids analysis, 100 μL of plasma extraction sample or 400 μL pull-down samples was used for DMAPA (dimethylaminopropylamine) derivatization for improving MS sensitivity of free fatty acids. Prior to the derivatization, 50 ng of AA-d8 (deuterated arachidonic acid –d8) as the internal standard was added to each sample. The solvents in the sample were dried under a stream of nitrogen. To derivatize the sample, 50 μL of 50 mM EDC (N-ethyl-N-(3-dimethylaminopropyl)-carbodiimide hydrochloride) and 50 mM DMAPA and 50 mM DMAP (4-dimethylaminopyridine) were added to the dried fatty acid samples and heated at 50 ◦C for 30 minutes. The samples were dried under nitrogen and then dissolved in 1 mL of ethanol : water for MS. |