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MB Sample ID: SA052766
| Local Sample ID: | Control_1.1 |
| Subject ID: | SU000935 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Cell Biosource Or Supplier: | ATCC |
| Cell Strain Details: | Daoy (ATCC® HTB-186™) |
| Subject Comments: | cancer cell line |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000942 |
| Sampleprep Summary: | The samples were thawed at room temperature and subjected to centrifugation for 17 min at 3000 rpm and 4°C. A quality control (QC) sample was created by pooling an equal volume from all samples. The aqueous supernatants were transferred to clean extraction tubes followed by addition of chloroform and methanol for the final proportion 2.85:4:4 water:methanol:chloroform. The extraction tubes were gently vortexed and then stored at 8°C for 20 min prior to centrifugation for 20 min at 3000 rpm and 4°C. The aqueous phases were recovered and evaporated to dryness at 40°C under N2. All samples were stored at -80°C after evaporation. Prior to analysis the samples were reconstituted in acetonitrile:Milli-Q water 90:10 |
| Processing Method: | lysis, freeze thaw cycles and sonication (see cell harvesting) |
| Processing Storage Conditions: | Room temperature and 8°C during extraction, -80°C before and after extraction |
| Extraction Method: | Liquid Liquid Extraction using CHCl3:MeOH:H2O 4:4:2.85 |
| Extract Storage: | Extracts were stored in -80°C after evaporation |
| Sample Resuspension: | Acetonitrile:Milli-Q water 90:10 |
| Cell Type: | Daoy cells |
