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MB Sample ID: SA054620
Local Sample ID: | TM3 |
Subject ID: | SU000958 |
Subject Type: | Rat |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-dawley |
Age Or Age Range: | 8 weeks |
Weight Or Weight Range: | 300-400 g |
Gender: | male |
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Sample Preparation:
Sampleprep ID: | SP000965 |
Sampleprep Summary: | Prior to analysis, lipids and small non-polar metabolites were separated from proteins in blood using isopropyl alcohol (IPA) for protein precipitation, shown to be advantageous over the Folch or Bligh and Dyer methods for a variety of experimental considerations.40-42 For protein precipitation purposes, serum samples were thawed on ice for 1 h and serum was mixed 1:3 v/v with IPA. Samples were centrifuged at 16,000 x g for 7 min, and the supernatant was collected. Samples were stored at -80 °C until analysis. |