Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA054635

Local Sample ID:	TH7
Subject ID:	SU000958
Subject Type:	Rat
Subject Species:	Rattus norvegicus
Taxonomy ID:	10116
Genotype Strain:	Sprague-dawley
Age Or Age Range:	8 weeks
Weight Or Weight Range:	300-400 g
Gender:	male

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP000965
Sampleprep Summary:	Prior to analysis, lipids and small non-polar metabolites were separated from proteins in blood using isopropyl alcohol (IPA) for protein precipitation, shown to be advantageous over the Folch or Bligh and Dyer methods for a variety of experimental considerations.40-42 For protein precipitation purposes, serum samples were thawed on ice for 1 h and serum was mixed 1:3 v/v with IPA. Samples were centrifuged at 16,000 x g for 7 min, and the supernatant was collected. Samples were stored at -80 °C until analysis.

Sampleprep ID:

SP000965

Sampleprep Summary:

Prior to analysis, lipids and small non-polar metabolites were separated from proteins in blood using isopropyl alcohol (IPA) for protein precipitation, shown to be advantageous over the Folch or Bligh and Dyer methods for a variety of experimental considerations.40-42 For protein precipitation purposes, serum samples were thawed on ice for 1 h and serum was mixed 1:3 v/v with IPA. Samples were centrifuged at 16,000 x g for 7 min, and the supernatant was collected. Samples were stored at -80 °C until analysis.