Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA070177

Local Sample ID:	62C
Subject ID:	SU001081
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001088
Sampleprep Summary:	Frozen serum samples were thawed on ice, and 300 µL of extraction solution (acetone: acetonitrile: methanol 1:1:1, cooled to -20 ˚C) was added to 100 µL of serum. Samples were vortex-mixed for 20 s and centrifuged at 16,000 × g for 5 min to pellet proteins. To remove most lipids and other non-polar metabolites, 800 µL of dichloromethane was added to ~350 µL of supernatant and vortex-mixed. Following the addition of 250 µL deionized water, samples were vortex-mixed and kept on ice for 10 min. The aqueous phase was subject to FI-TWIM-MS analysis. Sample blanks were prepared using deionized water instead of serum following the same procedure.

Sampleprep ID:

SP001088

Sampleprep Summary:

Frozen serum samples were thawed on ice, and 300 µL of extraction solution (acetone: acetonitrile: methanol 1:1:1, cooled to -20 ˚C) was added to 100 µL of serum. Samples were vortex-mixed for 20 s and centrifuged at 16,000 × g for 5 min to pellet proteins. To remove most lipids and other non-polar metabolites, 800 µL of dichloromethane was added to ~350 µL of supernatant and vortex-mixed. Following the addition of 250 µL deionized water, samples were vortex-mixed and kept on ice for 10 min. The aqueous phase was subject to FI-TWIM-MS analysis. Sample blanks were prepared using deionized water instead of serum following the same procedure.