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MB Sample ID: SA070181
Local Sample ID: | 45H |
Subject ID: | SU001081 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
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Sample Preparation:
Sampleprep ID: | SP001088 |
Sampleprep Summary: | Frozen serum samples were thawed on ice, and 300 µL of extraction solution (acetone: acetonitrile: methanol 1:1:1, cooled to -20 ˚C) was added to 100 µL of serum. Samples were vortex-mixed for 20 s and centrifuged at 16,000 × g for 5 min to pellet proteins. To remove most lipids and other non-polar metabolites, 800 µL of dichloromethane was added to ~350 µL of supernatant and vortex-mixed. Following the addition of 250 µL deionized water, samples were vortex-mixed and kept on ice for 10 min. The aqueous phase was subject to FI-TWIM-MS analysis. Sample blanks were prepared using deionized water instead of serum following the same procedure. |