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MB Sample ID: SA074536
Local Sample ID: | sample.6 |
Subject ID: | SU001138 |
Subject Type: | Cultured cells |
Subject Species: | Mus musculus |
Taxonomy ID: | 10090 |
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Sample Preparation:
Sampleprep ID: | SP001145 |
Sampleprep Summary: | The hydrophobic fraction was extracted from the samples using an organic liquid-liquid extraction technique with methyl tert-butyl ether (MTBE) and water (Cruickshank-Quinn et al. 2014; Yang et al. 2013). The upper hydrophobic layer was transferred to a new tube. The hydrophobic layer was then dried under nitrogen, reconstituted in 200 µL of methanol, and stored at -80 °C until LCMS analysis. |
Sampleprep Protocol Filename: | AKB_Targeted-protocol.docx |
Sampleprep Protocol Comments: | For AKB study 1 protocol, the collection, treatment, and sample prep are all in the same file. |