Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA074557

Local Sample ID:	12C-AC-Leaf
Subject ID:	SU001139
Subject Type:	Plant
Subject Species:	Allium cepa;Arabidopsis thaliana;Glycine max;Glycyrrhiza glabra
Subject Comments:	Allium cepa/Arabidopsis thaliana/Glycine max/Glycyrrhiza glabra/Glycyrrhiza urahensis/Medicago truncatula/Nicotiana tabacum/Ophiorrhiza pumila/Oryza sativa/Solanum lycopersicum/Solanum tuberosum/Zea mays

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Sample Preparation:

Sampleprep ID:	SP001146
Sampleprep Summary:	Samples (3 mg) of dried plant tissues were weighted in a 2.0-ml microtube (Sarstedt). Their metabolites were extracted with 150 µl of 80% MeOH containing 2.5 µM lidocaine and 10-camphour sulfonic acid per mg of dry weight using a mixer-mill with zirconia beads [7 min at 18 Hz and 4 degree C (MM300; Retsch)]. After 10-min centrifugation at 17,800 g the supernatant was filtered using an HLB µElution plate (Waters).

Sampleprep ID:

SP001146

Sampleprep Summary:

Samples (3 mg) of dried plant tissues were weighted in a 2.0-ml microtube (Sarstedt). Their metabolites were extracted with 150 µl of 80% MeOH containing 2.5 µM lidocaine and 10-camphour sulfonic acid per mg of dry weight using a mixer-mill with zirconia beads [7 min at 18 Hz and 4 degree C (MM300; Retsch)]. After 10-min centrifugation at 17,800 g the supernatant was filtered using an HLB µElution plate (Waters).