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MB Sample ID: SA075741

Local Sample ID:Standard Food
Subject ID:SU001155
Subject Type:Insect
Subject Species:Drosophila melanogaster
Taxonomy ID:7227

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Sample Preparation:

Sampleprep ID:SP001162
Sampleprep Summary:Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization.
Sampleprep Protocol Filename:SOP_blood-GCTOF-11082012_332.pdf
Sampleprep Protocol Comments:Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3.
Sample Derivatization:Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C.
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