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MB Sample ID: SA080282
Local Sample ID: | NR1 |
Subject ID: | SU001220 |
Subject Type: | Plant |
Subject Species: | Phoenix dactylifera |
Taxonomy ID: | 42345 |
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Sample Preparation:
Sampleprep ID: | SP001228 |
Sampleprep Summary: | A total of 24 samples were analyzed using LC-MS by HILIC chromatography on a 0.5 x 150 mm Zic-pHILIC column using 10 mM NH4HCO3 in water (A) and 10 mM NH4HCO3 in 95% acetonitrile (B) as solvents and by RPLC chromatography using the same solvents and a 0.5 x 100 mm PLRPS column. The Samples were extracted at a tissue concentration of 100 mg/mL (leaves) or 50 mg/mL (roots) in 80% methanol by vortexing for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. Samples were injected onto the LC-MS and data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra. |