Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA080305

Local Sample ID:	MTLZ-3
Subject ID:	SU001221
Subject Type:	Plant
Subject Species:	Phoenix dactylifera
Taxonomy ID:	42345

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Sample Preparation:

Sampleprep ID:	SP001229
Sampleprep Summary:	Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.

Sampleprep ID:

SP001229

Sampleprep Summary:

Metabolomics and basic data analysis were carried out for service charges at proteomics and mass spectrometry facility, Donald Danforth Plant Science Centre, Sant Louis, USA, using liquid chromatography mass spectrometry. Briefly, a total of 24 samples were analyzed for polar metabolites using hydrophilic interaction liquid chromatography (HILIC) and nonpolar metabolites using reverse phase liquid chromatography (RPLC). The Samples were extracted in 80% methanol at a tissue concentration of 100 mg/mL by a vortex for 10 minutes. The solids were collected by centrifugation, and then the supernatant was filtered through a 0.8 μm spin filter. The extracts were injected into the LC-MS and the data was acquired in polarity switching mode with data dependent acquisition of MS/MS spectra.