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MB Sample ID: SA080483
Local Sample ID: | 0-3_256_1_vq |
Subject ID: | SU001227 |
Subject Type: | Other |
Subject Species: | Homo sapiens |
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Sample Preparation:
Sampleprep ID: | SP001235 |
Sampleprep Summary: | Samples were prepared for metabolomics analysis using established methods(Johnson et al. (2010). Analyst; Go et al. (2015). Tox Sci). Prior to analysis, plasma aliquots were removed from storage at -80 degrees C and thawed on ice. Each cryotube was then vortexed briefly to ensure homogeneity, and 50 microliters was transferred to a clean microfuge tube. Immediately after, the plasma was treated with 100 microliters of ice-cold LC-MS grade acetonitrile(Sigma Aldrich) containing 2.5 microliters of internal standard solution with eleven stable isotopic chemicals selected to cover a range of chemical properties. Following addition of acetonitrile, samples were equilibrated for 30 min on ice, upon which precipitated proteins were removed by centrifuge (14,000rpm at 4 degrees C for 10 min). The resulting supernatant (100 microliters) was removed, added to a low volume autosampler vial and maintained at 4 degrees C until analysis (<22 h). |