Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA080836

Local Sample ID:	B49
Subject ID:	SU001234
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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MS

Sample Preparation:

Sampleprep ID:	SP001242
Sampleprep Summary:	The serum samples were thawed at room temperature and 100 μL was pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 300 μL of methanol, and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were vortexed for 30 s and centrifuged at 12000 rpm for 15 minat 4 °C. 200 μL of the supernatant was transferred to a vial for further analysis.

Sampleprep ID:

SP001242

Sampleprep Summary:

The serum samples were thawed at room temperature and 100 μL was pipetted into centrifuge tubes (1.5 mL) in preparation for extraction. The protein was precipitated with 300 μL of methanol, and 10 μL of internal standard (2.9 mg/mL, DL-o-Chlorophenylalanine) was added. The samples were vortexed for 30 s and centrifuged at 12000 rpm for 15 minat 4 °C. 200 μL of the supernatant was transferred to a vial for further analysis.