Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA081275

Local Sample ID:	8
Subject ID:	SU001237
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Gender:	Female

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP001245
Sampleprep Summary:	Serum samples were thawed on ice and subject to two different sample preparation protocols to profile either non-polar or polar sub-metabolomes. Reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC) UPLC-MS analysis were used for these different polarity metabolite fractions to obtain complementary information. Methanol (for polar) or iso-propanol (for non-polar) was added to a 50 μL serum sample in a 3:1 ratio to precipitate proteins. Samples were vortex mixed for 10 s and centrifuged at 13,000 rpm for 7 min. Then, 150 μL supernatant was frozen at -80 °C for UPLC-MS analysis. A sample blank was prepared with 50 μL of LC-MS grade water, and a pooled quality control (QC) sample was created by mixing 10 μL aliquot of each serum sample. Both the sample blank and the pooled sample were processed with the same procedure as the murine serum samples. Solvent blanks and sample blanks were analyzed together with murine serum samples.

Sampleprep ID:

SP001245

Sampleprep Summary:

Serum samples were thawed on ice and subject to two different sample preparation protocols to profile either non-polar or polar sub-metabolomes. Reverse phase (RP) and hydrophilic interaction liquid chromatography (HILIC) UPLC-MS analysis were used for these different polarity metabolite fractions to obtain complementary information. Methanol (for polar) or iso-propanol (for non-polar) was added to a 50 μL serum sample in a 3:1 ratio to precipitate proteins. Samples were vortex mixed for 10 s and centrifuged at 13,000 rpm for 7 min. Then, 150 μL supernatant was frozen at -80 °C for UPLC-MS analysis. A sample blank was prepared with 50 μL of LC-MS grade water, and a pooled quality control (QC) sample was created by mixing 10 μL aliquot of each serum sample. Both the sample blank and the pooled sample were processed with the same procedure as the murine serum samples. Solvent blanks and sample blanks were analyzed together with murine serum samples.