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MB Sample ID: SA083091
| Local Sample ID: | LO-3 |
| Subject ID: | SU001261 |
| Subject Type: | Plant |
| Subject Species: | Ginkgo Biloba |
| Taxonomy ID: | 3311 |
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Sample Preparation:
| Sampleprep ID: | SP001269 |
| Sampleprep Summary: | Andrographolide was dissolved in acetonitrile as an internal standard solution with the terminal concentration of 133μM. Of each leaf sample, 200mg liquid nitrogen-grinded powder was homogenized in 30ml 70% ethanol solution (v: v= 70: 30) followed by 1 min vortex and 1 h ultrasound extraction as previously described with minor modifications (Yu, Lai et al. 2003, Tohge, Nishiyama et al. 2005, Zhou, Yao et al. 2014). After a 13000rpm centrifugation for 10min at 4℃, 1ml supernatant of the solution was transferred and evaporated to dryness under nitrogen gas at 37℃ (Li, Guo et al. 2017). The residue was re-dissolved in 1ml acetonitrile and centrifuged at 13000rpm for 10 min at 4℃. 950μL supernatant was transferred to mix with 50μL internal standard solution to be the final sample for further UPLC-QTOF/MS analysis. |
| Processing Storage Conditions: | Described in summary |
